| 1. | Recent progress in handmade somatic cell cloning
徒手切割法体细胞克隆研究进展 |
| 2. | The es cell clones decreased and disappeared gradually after 9 passages
本实验中的小鼠胚胎es细胞再传至第九代后逐渐消失。 |
| 3. | In terms of human cell cloning , he gained a great deal of experience
在人体活细胞（克隆）培养增殖技术方面积累了丰富的经验。 |
| 4. | Plasma cell clone
浆细胞纯系 |
| 5. | Of more immediate concern is how telomere length might affect human cells cloned to help fight diseases , rather than cloning an entire human
目前更紧迫的问题不是克隆人，而是要搞清端粒长度如何影响克隆的人体细胞，从而帮助治疗疾病。 |
| 6. | The plasmid ploxgfp dna was transfected into bovine fetal fibroblast cells , then on the selected g418 medium , the green fluorescent cell clones were gained
将鸡-干扰素cdna克隆到载体plox上的loxp和lox511序列之间，构建成另一打靶载体? ploxifn 。 |
| 7. | ( 4 ) the mrna and protein expression grp94 were detected by rt - pcr and western bloting respectively to find the cell clones that have been transfected successfully . 2
( 4 )分别通过rt - pcr方法和westernblot方法从mrna和蛋白水平检测grp94的表达情况，以此鉴定转染成功的阳性克隆株。 |
| 8. | There was about 7 days interval between every two passages . eg cell clones can be found in each primary culture from those embryos of appropriate age . in culture of one embryonic cell line , eg cell clones maintained after 9 passages
在实验中，几个适龄的胚胎的原代培养物中都出现了鸟巢状的eg细胞集落，维持时间最长的一个胚胎细胞株在传代培养了9代之后仍然可以观察到eg细胞集落。 |
| 9. | The two mediums can meet primary culture and passage culture of the black bear fibroblast cells . the method of single cell cloning by micro - manipulating purifies fibroblast cells . as a result , steady fibroblast cells can be obtained and the rates of the cloning formation is 93 . 75 %
通过单细胞集落显微法纯化细胞，可克服单细胞克隆中因细胞密度太低，难以形成克隆的缺点，而且操作简单，效率高，速度快，其克隆率可达93 . 75 。 |
